Journal: Advanced Science
Article Title: Retinoic Acid Reprograms Mast Cells Toward a Proinflammatory State to Enhance Antitumor Immunity
doi: 10.1002/advs.202509340
Figure Lengend Snippet: RA–RARα signaling induces CIITA‐dependent antigen‐presenting programming in mast cells. A) Flow cytometry analysis showing HLA‐DR expression after IFN‐γ +TTNPB and IFN‐γ +Bexarotene treatment for 48 h ( n = 3). B) Flow cytometry analysis showing CD40 expression after IFN‐γ +TTNPB and IFN‐γ +Bexarotene treatment for 48 h ( n = 3). C) Flow cytometry analysis showing HLA‐DR expression after IFN‐γ +AM580, IFN‐γ +Adapalene, and IFN‐γ +Palovarotene treatment for 48 h ( n = 3). D) Flow cytometry analysis showing CD40 expression after IFN‐γ +AM580, IFN‐γ +Adapalene, and IFN‐γ +Palovarotene treatment for 48 h ( n = 3). E) Flow cytometry analysis showing HLA‐DR expression after IFN‐γ +RA, IFN‐γ + RA+ Ro 41‐5253 treatment for 48 h ( n = 3). F) Flow cytometry analysis showing CD40 expression after IFN‐γ +RA, IFN‐γ + RA+ Ro 41‐5253 treatment for 48 h ( n = 3). G) Flow cytometry analysis showing CMV antigen presentation by mast cells to CD4⁺ and CD8⁺ T cells under different treatments, with T cell activation assessed by 4‐1BB and TNF‐α expression. H) Volcano plot showing significantly differentially expressed genes between the IFN‐γ + RA treatment group and the IFN‐γ treatment alone group. I) The mRNA expression of CIITA after IFN‐γ, RA, IFN‐γ +RA, IFN‐γ + RA+ Ro 41‐5253 treatment for 48 h ( n = 3).
Article Snippet: The cells were then treated with 100 U mL −1 IFN‐γ (Propretech, #300‐02) (IFN‐γ group) or 100 U mL −1 IFN‐γ plus 50 nM RA (MeilunBio, #MB1302) (IFN‐γ + RA group) for 48 or 72 h. TTNPB (#HY‐15682), Bexarotene (#HY‐14171), AM580 (#HY‐10475), Adapalene (#HY‐B0091), Palovarotene (#HY‐14799), Ro 41‐5253 (#HY‐116248) were purchased from MCE.
Techniques: Flow Cytometry, Expressing, Immunopeptidomics, Activation Assay
